CAPACITY OF CIRCULATING NEUTROPHILS TO PRODUCE REACTIVE OXYGEN SPECIES AFTER EXHAUSTIVE EXERCISE

Citation
K. Suzuki et al., CAPACITY OF CIRCULATING NEUTROPHILS TO PRODUCE REACTIVE OXYGEN SPECIES AFTER EXHAUSTIVE EXERCISE, Journal of applied physiology, 81(3), 1996, pp. 1213-1222
Citations number
31
Categorie Soggetti
Physiology,"Sport Sciences
ISSN journal
87507587
Volume
81
Issue
3
Year of publication
1996
Pages
1213 - 1222
Database
ISI
SICI code
8750-7587(1996)81:3<1213:COCNTP>2.0.ZU;2-S
Abstract
To investigate the cause of disagreement within the large body of lite rature concerning the effect of exercise on the capacity of circulatin g neutrophils to produce reactive oxygen species (ROS), 10 male endura nce-trained athletes underwent maximal exercise. The generation of sup eroxide radical (O-2(-).) by neutrophils was first detected on a cell- by-cell basis by using histochemical nitro blue tetrazolium tests perf ormed directly on fresh unseparated blood, which showed that responsiv e neutrophils under several stimulatory conditions relatively decrease d after exercise. Similarly, O--(2). detected with bis-N-methylacridin ium nitrate (lucigenin)-dependent chemiluminescence (CL) of a fixed nu mber of purified neutrophils on stimulation with opsonized zymosan was decreased slightly after exercise. In contrast, the 5-amino-2,3-dihyd ro-1,4-phthalazinedione (luminol)dependent CL response of the neutroph ils indicative of the myeloperoxidase (MPO)-mediated formation of high ly reactive oxidants was significantly enhanced after exercise. It the refore suggests that the pathway of neutrophil ROS metabolism might be forwarded from the precursor O-2(-). production to the stages of more reactive oxidant formation due to the facilitation of MPO degranulati on. In addition, these phenomena were closely associated with the exer cise-induced mobilization of neutrophils from the marginated pool into the circulation, which was mediated by the overshooting of catecholam ines during exercise. These findings indicate that the use of differen t techniques for detecting ROS or the different stages of neutrophil R OS metabolism could explain some of the disparate findings of the prev ious studies.