A STRATEGY FOR THE IDENTIFICATION OF 2'-DEOXYNUCLEOSIDE AND 2'-DEOXYNUCLEOTIDE ADDUCTS USING ELECTROSPRAY TANDEM MASS-SPECTROMETRY

A methodology using electrospray tandem mass spectrometry (ES MS/MS) w as developed to identify 2'-deoxynucleotide adducts of bisphenol A dig lycidylether (BPADGE). The 2'-deoxynucleotide adducts were formed in v itro and were separated using reversed-phase high-performance liquid c hromatography (RP-HPLC). The adducts were investigated by (-) electros pray liquid chromatography-mass spectrometry [(-) ES LC-MS]. (-) ES LC -MS/MS allowed the differentiation between 5'-phosphate- and base-alky lated adducts. More detailed structural information on the heterocycli c moiety was obtained by examining the (+) ES LC-MS/MS data and by com parison with the (+) ES LC-MS/MS spectra of the corresponding 2'-deoxy nucleoside adducts.