Z. Rahim et Kms. Aziz, FACTORS AFFECTING PRODUCTION OF HEMOLYSIN BY STRAINS OF VIBRIO-FLUVIALIS, Journal of diarrhoeal diseases research, 14(2), 1996, pp. 113-116
The in vitro production of haemolysin by Vibrio fluvialis was studied
using sheep erythrocyte. The effect of the composition of various medi
a and different concentrations of sodium chloride on the production of
haemolysin and heat-stability was investigated. Comparatively higher
titre of haemolysin production was noted in brain heart infusion (BHI)
broth. Adding 0.5% NaCl to BHI broth reduced the production of haemol
ysin; adding 5.0% NaCl to the medium totally inhibited it. The highest
titre of haemolysin was produced at 30 degrees C and 37 degrees C, wh
ereas no haemolysin was produced at 50 degrees C. Haemolytic activity
was totally destroyed when heated at 56 degrees C for 30 minutes. Haem
olysin could be assayed easily following this method.