COMPARISON BETWEEN ANTIGEN-CAPTURE ELISA AND CONVENTIONAL METHODS USED FOR TITRATION OF INFECTIOUS BURSAL DISEASE VIRUS

Two antigen-capture enzyme-linked immunosorbent assays (polyclonal and monoclonal AC-ELISAs) were developed and evaluated for titration of i nfectious bursal disease viruses (IBDVs) propagated in different host systems, namely BGM-70 continuous cell line, primary chicken embryo fi broblast cells, and chicken bursa of Fabricius. The polyclonal system was more sensitive (P < 0.05) than the monoclonal system but both were specific as indicated by the negative results obtained with three non -IBDVs. The results revealed chat the conventional systems used for ti tration of IBDVs (cell cultures and embryonating chicken eggs) were mo re sensitive than the polyclonal AC-ELISA.