Cw. Tang et al., VISUALIZATION AND CHARACTERIZATION OF CCK RECEPTORS IN EXOCRINE PANCREAS OF RAT WITH STORAGE PHOSPHOR AUTORADIOGRAPHY, Pancreas, 13(3), 1996, pp. 311-315
Studies on cholecystokinin (CCK) receptors in rat pancreas are usually
performed on homogenates. Using storage phosphor autoradiography, a n
ew imaging technique with a high sensitivity and large linear dynamic
range, we visualized and characterized CCK receptors in tissue section
s of normal rat pancreas. The density of CCK receptors in pancreatic t
issue sections from 10 normal rats appeared to be unevenly distributed
and variable in serial sections. The binding of labeled CCK-8 was mar
kedly inhibited by CCK-8 and CCK-A receptor antagonists, but it was on
ly weakly affected by gastrin and CCK-B receptor antagonists. At room
temperature the CCK-8 dose-inhibition curve was fitted by a two-site m
odel: one with a high-affinity but low-capacity site and another with
a low-affinity but high-capacity site. The CCK-8 dose-inhibition curve
showed that the inhibition of the variable high-density receptors too
k place at a low concentration of CCK-8, while the diffuse low-density
receptors were inhibited at the high concentration of 1 mu M CCK-8. B
inding of labeled CCK-8 at 37 degrees C was homogeneous with a low aff
inity and comprised only 4% of that found at room temperature. In summ
ary, an uneven density of CCK receptors in the rat exocrine pancreas w
as observed and attributed to the variable expression of high-affinity
CCK receptors in pancreatic acini.