THE DIELECTRIC-PROPERTIES OF UNFROZEN WATER IN CARP MYOFIBRILS UNDER FROZEN CONDITIONS

The dielectric properties of carp myofibrils in the presence and absen ce of electrolytes under frozen conditions were measured. Unfrozen wat er was characterized by the apparent dielectric relaxation time (tau) and apparent activation energy calculated. The unfrozen water was divi ded into three groups that had different relaxation mechanisms by a Co le-Cole plot. The temperature-dependence (reciprocal of absolute tempe rature) of tau (Arrhenius plot) presented a biphasic pattern with a br eaking point at -15.3 and -23.2 degrees C in the absence and presence of electrolytes, respectively. In the temperature range from the break ing point to the lowest temperature examined, it seems that the unfroz en water is ''unfreezable water'' and corresponds to monolayer water. At the same time, it is considered that unfrozen water in the temperat ure range from the breaking point to the highest temperature examined corresponds to the bound water of multilayer water. The mobility of un frozen and unfreezable waters increased in the presence of electrolyte s under the temperatures examined.