DETECTION OF CANINE PARVOVIRUS ANTIGENS WITH ANTIBODIES TO SYNTHETIC PEPTIDES

Antibodies produced in rabbits against an 18-amino acid peptide (pepti de 1, NSLPQSEGATNFGDIGVP) of capsid protein VP2/residues 292-309 of ca nine parvovirus (CPV) or against an 18-amino acid peptide (peptide 2, GKRNTVLFHGPASTKGKS) of nonstructural protein NS1/residues 391-409 of C PV identified, in immunofluorescence analysis, viral antigens in canin e A 72 cells infected with CPV. Antibodies to peptide 2 also identifie d viral antigens in bovine cells infected with bovine parvovirus. In w estern blot analysis, antibodies to peptide 1. and peptide 2 also dete cted viral antigens derived from blue fox parvovirus, feline parvoviru s, mink enteritis virus and raccoon dog parvovirus. The peptide antibo dies could be used as convenient tools in diagnosis of infections caus ed by CPV or closely related viruses affecting cats, minks, blue foxes and raccoon dogs.