Us. Muchhal et al., PHOSPHATE TRANSPORTERS FROM THE HIGHER-PLANT ARABIDOPSIS-THALIANA, Proceedings of the National Academy of Sciences of the United Statesof America, 93(19), 1996, pp. 10519-10523
Two cDNAs (AtPT1 and AtPT2) encoding plant phosphate transporters have
been isolated from a library prepared with mRNA extracted from phosph
ate-starved Arabidopsis thaliana roots, The encoded polypeptides are 7
8% identical to each other and show high degree of amino acid sequence
similarity with high-affinity phosphate transporters of Saccharomyces
cerevisiae, Neurospora crassa, and the mycorrhizal fungus Glomus vers
iforme. The AtPT1 and AtPT2 polypeptides are integral membrane protein
s predicted to contain 12 membrane-spanning domains separated into two
groups of six by a large charged hydrophilic region, Upon expression,
both AtPT1 and AtPT2 were able to complement the pho84 mutant phenoty
pe of yeast strain NS219 lacking the high-affinity phosphate transport
activity, AtPT1 and AtPT2 are representatives of two distinct, small
gene families in A. thaliana. The transcripts of both genes are expres
sed in roots and are not detectable in leaves. The steady-state level
of their mRNAs increases in response to phosphate starvation.