IMPROVED RAPD AMPLIFICATION OF RECALCITRANT PLANT DNA BY THE USE OF ACTIVATED-CHARCOAL DURING DNA EXTRACTION

An efficient procedure has been developed for DNA extraction from cott on by modifying the original CTAB method. The major improvement concer ns the use of activated charcoal to bind resinous and coloured compoun ds which copurify with the DNA. The efficiency of amplification by RAP D was used as a criterion to evaluate the action of activated charcoal . Twenty-five random decamers from Operon Technologies were used to co mpare DNA samples extracted in the presence or absence of activated ch arcoal. The results in terms of amplification suggest that the use of activated charcoal in DNA extraction enhances RAPD amplification. This technique was initially developed for cotton but it has been applied successfully to other recalcitrant plants such as coffee, rubber tree, cassava and banana.