Pg. Spizzirri et al., TIME-RESOLVED CONFOCAL FLUORESCENCE MICROSCOPY OF PORPHYRINS FOR PHOTOTHERAPY, Lasers in medical science, 11(4), 1996, pp. 237-246
The application of a novel time-resolved confocal fluorescence microsp
ectrometer to studies of the distribution and speciation of porphyrin
photosensitizers in rat C6 cerebral glioma cells is described. The ins
trument combines a mode-locked argon ion laser excitation source with
time-correlated single photon counting fluorescence detection and has
sub-micron spatial and sub-nanosecond temporal resolution, The porphyr
ins studied were haematoporphyrin derivative (HpD), haematoporphyrin I
X (HP), porphyrin c (Pc) and the tetrakiscarborane carboxylate ester o
f 2,4-(alpha, beta-dihydroxyethyl) deuteroporphyrin IX (BOPP), From th
e heterogeneous emission observed in vitro, assignments and spatial lo
cation of various porphyrin species are proposed.