A. Heredia et al., THE EFFECT OF TGF-BETA-1 ON CELL-PROLIFERATION AND PROTEOGLYCAN PRODUCTION IN HUMAN-MELANOMA CELLS DEPENDS ON THE DEGREE OF CELL-DIFFERENTIATION, Cancer letters, 109(1-2), 1996, pp. 39-47
The effect of transforming growth factor beta 1 (TGF-beta 1) on cell p
roliferation, colony formation in soft agar and synthesis and structur
e of proteoglycans was studied in three human melanoma cell lines at d
ifferent stages of differentiation: SK-mel-1.36-1-5 (early), SK-mel-3.
44 (intermediate) and SK-mel-23 (late). TGF-beta 1 potently inhibited
cell growth in monolayer as well as in soft agar. TGF-beta 1 increased
the release of sulfated proteoglycans into the medium, including the
cell-specific melanoma proteoglycan, mel-PG, and induced changes in di
saccharide composition and sulfation of the glycosaminoglycan chains.
In all the cases, the effect of TGF-beta 1 was more pronounced in the
most undifferentiated cell line SK-mel-1.36-1-5 than in the SK-mel-3.4
4, whereas it had no effect on the most differentiated SK-mel-23 cells
.