THE EFFECT OF TGF-BETA-1 ON CELL-PROLIFERATION AND PROTEOGLYCAN PRODUCTION IN HUMAN-MELANOMA CELLS DEPENDS ON THE DEGREE OF CELL-DIFFERENTIATION

Authors
HEREDIA A VILLENA J ROMARIS M MOLIST A BASSOLS A
Citation
A. Heredia et al., THE EFFECT OF TGF-BETA-1 ON CELL-PROLIFERATION AND PROTEOGLYCAN PRODUCTION IN HUMAN-MELANOMA CELLS DEPENDS ON THE DEGREE OF CELL-DIFFERENTIATION, Cancer letters, 109(1-2), 1996, pp. 39-47
Citations number
22
Categorie Soggetti
Oncology
Journal title
Cancer letters → ACNP
ISSN journal
03043835
Volume
109
Issue
1-2
Year of publication
1996
Pages
39 - 47
Database
ISI
SICI code
0304-3835(1996)109:1-2<39:TEOTOC>2.0.ZU;2-Q
Abstract
The effect of transforming growth factor beta 1 (TGF-beta 1) on cell p roliferation, colony formation in soft agar and synthesis and structur e of proteoglycans was studied in three human melanoma cell lines at d ifferent stages of differentiation: SK-mel-1.36-1-5 (early), SK-mel-3. 44 (intermediate) and SK-mel-23 (late). TGF-beta 1 potently inhibited cell growth in monolayer as well as in soft agar. TGF-beta 1 increased the release of sulfated proteoglycans into the medium, including the cell-specific melanoma proteoglycan, mel-PG, and induced changes in di saccharide composition and sulfation of the glycosaminoglycan chains. In all the cases, the effect of TGF-beta 1 was more pronounced in the most undifferentiated cell line SK-mel-1.36-1-5 than in the SK-mel-3.4 4, whereas it had no effect on the most differentiated SK-mel-23 cells .