The combination of photodynamic therapy (PDT) and the microtubule (MT)
inhibitor, vincristine (VCR) or taxol, was studied in the CaD2 mammar
y tumour model in mice. Meso-tetra(di-adjacent-sulphonatophenyl) porph
ine (TPPS2a) was used as a photosensitizer. An enhanced antitumour eff
ect was found when VCR, at an almost non-toxic dose (1 mg/kg), was inj
ected i.p. into the mice 6 h before PDT, while no such enhanced effect
was observed when the same dose of VCR was given either 12 or 24 h be
fore PDT or immediately before PDT. Furthermore, it was found that the
number of mitotic cells increased 4-5-fold 6 h after the injection of
VCR into the mice. VCR did not enhance the sensitivity of normal skin
to PDT. Combination of PDT and taxol was also studied. The antitumour
activity of PDT could be increased by taxol when the drug (35 mg/kg)
was administered i.p. either 6 h prior to PDT or immediately after or
before PDT. No significant enhancement in PDT efficiency was found whe
n PDT with photofrin was combined with VCR.