TOXICITY OF ETHANOL IN LOW CONCENTRATIONS - EXPERIMENTAL EVALUATION IN CELL-CULTURE

Citation
E. Tapani et al., TOXICITY OF ETHANOL IN LOW CONCENTRATIONS - EXPERIMENTAL EVALUATION IN CELL-CULTURE, Acta radiologica, 37(6), 1996, pp. 923-926
Citations number
29
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging
Journal title
ISSN journal
02841851
Volume
37
Issue
6
Year of publication
1996
Pages
923 - 926
Database
ISI
SICI code
0284-1851(1996)37:6<923:TOEILC>2.0.ZU;2-O
Abstract
Purpose: To define the threshold ethanol concentration that is toxic t o cultured cells. Methods: Three malignant cell lines and freshly isol ated normal rat hepatocytes were exposed to 0-50% (vol.) ethanol (conc entrations used were 0, 5, 10, 15, 20, 25, 30, 40 and 50%) on tissue c ulture plates for 0.25-60 min (exposure times used were 0.25, 5, 10, 2 0, 30, 40. 50 and 60 min). Cytotoxicity was estimated by trypan blue e xclusion test and from H-3-thymidine incorporation. Results: All cells were killed by a 15-s exposure to 30-40% ethanol while a concentratio n as low as 15-20% gave a total response after 5-10-min exposures. Aft er a one-hour exposure of F9 carcinoma cells and hepatocytes, a total or nearly total response was achieved with 10% ethanol. The cytotoxic effect was thus dependent both on the exposure time and on the concent ration of ethanol. There were no significant differences in ethanol to lerance among the cell types. Conclusion: Ethanol seemed to kill cells in the cell culture effectively in much lower concentrations than tho se currently used in tumour ablation.