C. Arcelloni et al., DETERMINATION OF CEFTAZIDIME CONCENTRATION IN MUELLER-HINTON AGAR BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 742(1-2), 1996, pp. 121-126
Citations number
23
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A simple and rapid RP-HPLC method for the direct determination of ceft
azidime (a beta-lactam antibiotic) in agar (Mueller Hinton Agar-II) wa
s developed. The method, characterised by good precision (C.V.less tha
n or equal to 7.9%) and linearity in the 5-200 mu g/ml range (r(2) gre
ater than or equal to 0.998), showed high recovery from agar (104+/-8%
) and a sensitivity limit of 1.4 mu g/ml. The analytical procedure all
owed the determination of the ''true'' antibiotic concentration in the
agar matrix. In addition, the small volume sample may allow a precise
evaluation of the antibiotic levels point by point in the agar plates
necessary to study the kinetics of diffusion of ceftazidime.