DETERMINATION OF BIOLOGICALLY-ACTIVE CONSTITUENTS IN CENTELLA-ASIATICA

A high-performance liquid chromatographic method is described, using a n octadecylsilylated silica column, for simultaneous quantitative dete rmination of the bioactive terpene acids: asiatic acid, madecassic aci d and their respective glycosides, asiaticoside and madecassoside, in the plant Centella asiatica. The method is suitable for estimating the se compounds in plant material. The method is also suitable for the ro utine assay of pharmaceutical preparations containing a C. asiatica ex tract.