MOLECULAR-CLONING AND TISSUE-SPECIFIC EXPRESSION OF MOUSE KIDNEY 6-PHOSPHOFRUCTO-2-KINASE FRUCTOSE-2,6-BISPHOSPHATASE/

A 1932 bp cDNA clone encoding a novel isozyme of phosphofructo-2-kinas e/fructose-2,6-bisphosphatase (PFK-2/FBPase-2) was isolated from a mou se kidney cDNA library, The sequence encodes 519 amino acids and, base d on homology to rat heart genomic sequence, appears to be the product of alternative splicing from PFK-2/FBPase-2 gene B with an extended v ersion of exon 15. Northern blot analysis indicated that this clone co rresponds to an 8 kb mRNA expressed in multiple tissues, with the stro ngest signal in kidney, and detects several additional transcripts whi ch may be alternatively spliced from gene B.