Mj. Bogusz et al., APPLICABILITY OF VARIOUS BRANDS OF MIXED-PHASE EXTRACTION COLUMNS FOROPIATE EXTRACTION FROM BLOOD AND SERUM, Journal of chromatography B. Biomedical applications, 683(2), 1996, pp. 177-188
Citations number
34
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
Four commercially available types of mixed-phase solid-phase extractio
n (SPE) columns (Bond Elut Certify, Isolute Confirm HCX, Chromabond Dr
ug and Bakerbond Narc-2) were examined in order to compare the extract
ion efficiencies and chromatographic purity of extracts. The absolute
recovery of morphine, 6-monoacetylmorphine and codeine was examined in
blood and serum (ten samples each at two concentration levels), using
SPE columns of the same batch. GC-MS (ion trap) and HPLC with amperom
etric detection were used for quantitation. A distinct variability in
extraction recovery was observed among the same batches of all brands
of SPE columns. All extracts were chromatographically pure and no inte
rfering peaks were observed, neither in GC-MS nor in HPLC examinations
, but in some extracts large peaks of plasticizers were identified. Th
e measurements of how velocities of the same samples of blood or serum
through the SPE columns of the same batch showed very large variabili
ty of random character. The morphometric analysis of particles was per
formed for two batches of each sort of SPE columns by means of an imag
e analysing system. Symmetrical distribution of particle size was obse
rved only in Chromabond MN Drug packing, while in other cartridges lar
ge fractions of fine particles and nonhomogenous distribution were fou
nd. Only in one case the morphometric findings were pretty concordant
with the data available from the manufacturer; in two cases, observed
data varied considerably from that expected, and in one case no inform
ation was available at all. The study showed generally that there was
room for improvement in the quality of mixed-phase SPE columns.