Z00-FISH SUGGESTS A COMPLETE HOMOLOGY BETWEEN HUMAN AND CAPUCHIN MONKEY (PLATYRRHINI) EUCHROMATIN

Chromosome comparisons using in situ hybridization of all human chromo some-specific libraries on Capuchin monkey (Cebus capucinus, Cebidae, Platyrrhini) metaphases were performed with a new technique simultaneo usly revealing a G-banding and chromosome ''painting.'' A complete hom ology between human (HSA) and C. capucinus (CCA) chromosomes was demon strated, except for constitutive heterochromatin. Eleven C. capucinus chromosomes are homologous to 11 human chromosomes: CCA 2 = HSA 4; CCA 3 = HSA 6; CCA 12 = HSA 9; CCA 16 = HSA 11; CCA 10 = HSA 12; CCA 11 = HSA 13; CCA 20 = HSA 17; CCA 8 = HSA 19; CCA 23 = HSA 20; CCA 24 = HS A 22; and CCA X = HSA X. Ten C. capucinus chromosomes are homologous t o parts of human chromosomes: CCA 13 = HSA 8q; CCA 14 = HSA 2q; CCA 15 = HSA 1p + 1q proximal; CCA 17 = HSA 7 part; CCA 18 and 19 = HSA 3 pa rt; CCA 21 and 22 = HSA 1q distal; CCA 25 = HSA 10p; and CCA 26 = HSA 15q part. Six C. capucinus chromosomes are homologous to parts of two human chromosomes: CCA 1 = HSA 5 + 7 part; CCA 4 = HSA 2p + q proximal + 16q; CCA 5 = HSA 10q + 16p; CCA 6 = HSA 14 + 15 part; CCA 7 = HSA 8 p + 18; and CCA 9 = HSA 3 part + 21. Many previous banding comparisons were confirmed but several cryptic or complex rearrangements could be identified. With the C. capucinus karyotype having been shown to be f airly ancestral, this comparison opens the possibility to compare huma n chromosomes to most Cebidae species. (C) 1996 Academic Press, Inc.