M. Dinicola et al., COMBINED NEGATIVE AND POSITIVE SELECTION OF MOBILIZED CD34(-CELLS() BLOOD), British Journal of Haematology, 94(4), 1996, pp. 716-721
We tested four negative and two positive selection methods for separat
ion of CD34(+) cells from mobilized blood cells, and analysed fold-enr
ichment, purity and recovery of CD34(+) cells after selection procedur
es. The elimination of mature CD34(-) cells was achieved by adhesion t
o nylon-wool fibre (5 . 9+/-1 . 0 mean fold-enrichment and 65 . 2+/-2
. 3 mean recovery of CD34(+) cells), Standard or modified Ficoll-Hypaq
ue and Percoll density gradients, as well as phagocytosis with magneti
c beads, were less effective in eliminating CD34(-) cells, both purity
and fold-enrichment of CD34(+) cells being lower than those obtained
with separation by nylon-wool, Both positive selection methods tested,
Ceprate and MiniMacs System, generated highly purified CD34(+) cell p
opulations ranging from 80% to 90%, The recovery of CD34(+) cells was
optimal with MiniMacs (77 . 9+/-3 . 6) and low with Ceprate (28 . 8+/-
2 . 8). Based on these results, in two large-scale experiments we comb
ined nylon-wool fibre and MiniMacs System in a two-step separation pro
cedure obtaining a 36 . 9+/-2 . 6 mean fold-enrichment and a 50 . 5+/-
0 . 3 mean recovery of CD34(+) cells, In this way we achieved optimal
enrichment and recovery of CD34(+) cells, with a substantial saving of
cost compared to either selection method alone.