W. Bernhard et al., SYNTHESIS AND RELEASE OF PHOSPHATIDYLCHOLINE BY ISOLATED PORCINE GASTRIC MUCOUS CELLS IN PRIMARY CULTURE, European journal of clinical investigation, 26(9), 1996, pp. 797-802
Citations number
21
Categorie Soggetti
Medicine, Research & Experimental","Medicine, General & Internal
Phosphatidylcholine (PC) is the major phospholipid of the hydrophobic
gastric mucosal barrier and is chiefly released from mucous cells into
the gastric mucus. Whereas the mucosa contains highly unsaturated PC,
gastric mucus predominantly contains palmitoyl-oleoyl-PC and palmitoy
l-linoleoyl-PC, indicating a selective release of these PC species int
o the gastric lumen. In order to understand gastric PC metabolism, we
investigated synthesis and release of PC in cultivated porcine gastric
mucous cells, using dual labelling with [methyl-H-3]-choline and [1-C
-14]-palmitate, in the presence of 12-O-tetradecanoylphorbol-13-acetat
e (TPA), indomethacin and prostaglandin E(2) (PGE(2)). Linear incorpor
ation of [methyl-H-3]-choline and [1-C-14]-palmitate into PC was achie
ved for at least 8 h. In contrast to type II pneumocytes TPA increased
PC synthesis in gastric mucous cells but not its release. Indomethaci
n did not influence PC synthesis, but it decreased the release of newl
y synthesized PC. PGE(2) antagonized the effect of indomethacin on PC
release. We conclude that PC release by isolated porcine gastric mucou
s cells is regulated in a manner different from type II pneumocytes. P
C release is impaired by indomethacin and this impairment is restored
by PGE(2).