CHIMERISM ANALYSIS AFTER ALLOGENEIC BONE-MARROW TRANSPLANTATION WITH NONRADIOACTIVE RFLP AND PCR-AFLP USING THE SAME DNA

We describe a method combining RFLP and PCR-AFLP analyses for studying chimerism after allogeneic bone marrow transplantation. Using RFLP an alysis alone with DNA probe hMF1 87% of 244 donor/recipient pairs reve aled different specific bands. By examination of the identical DNA pro bes using PCR-AFLP, an additional 52% of the residual donor/recipient pairs could be identified. Thus, 94% of allogeneic transplantations ca n be monitored using a combination of RFLP and PCR-AFLP analyses at on e locus.