The stability in aqueous suspensions of two particulate celluloses, Si
gmacell type 100 and Avicel PH101, was analyzed. The effect of the pre
sence of a cellulase from Trichoderma reesei, ionic strength, and ultr
asonic agitation on the fragmentation/aggregation phenomena was studie
d. Particle size distributions of the powders were obtained with three
different particle sizers: the Galai CIS 100, the Coulter Multisizer
II, and the Malvern 2600c. The differences in the obtained absolute va
lues are discussed according to the measuring principles of each techn
ique; however, the overall conclusions are independent of the particle
sizer used. The enzyme breaks up the Avicel aggregates more effective
ly than ultrasound while the Sigmacell particles are stable under the
present experimental conditions. The stabilizing effect of celluloses
was tentatively explained using the DLVO (Derjaguin, Landau, Verweye,
and Overbeek) theory. The adsorbed enzyme did not change significantly
the zeta potential of the fibers, hence, the stabilizing effect was a
ttributed to a reduction in the attractive van der Waals forces and hy
dration effects. (C) 1997 by Elsevier Science Inc.