STABILIZATION OF DRY IMMOBILIZED ACETYLCHOLINESTERASE ON MICROTITRATION PLATES FOR COLORIMETRIC DETERMINATION OF ITS INHIBITORS IN WATER AND BIOLOGICAL-FLUIDS

Authors
NGUYEN VK EHRETSABATIER L GOELDNER M BOUDIER C JAMET G WARTER JM POINDRON P
Citation
Vk. Nguyen et al., STABILIZATION OF DRY IMMOBILIZED ACETYLCHOLINESTERASE ON MICROTITRATION PLATES FOR COLORIMETRIC DETERMINATION OF ITS INHIBITORS IN WATER AND BIOLOGICAL-FLUIDS, Enzyme and microbial technology, 20(1), 1997, pp. 18-23
Citations number
19
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Enzyme and microbial technology → ACNP
ISSN journal
01410229
Volume
20
Issue
1
Year of publication
1997
Pages
18 - 23
Database
ISI
SICI code
0141-0229(1997)20:1<18:SODIAO>2.0.ZU;2-#
Abstract
The ability of the gelatin (or BSA)-trehalose film to convert normally fragile dry immobilized acetylcholinesterase enzyme (AchE) into a sta ble reagent is shown. The remarkable properties of the dry immobilized AchE enzyme preparation are its stability to prolonged exposure to te mperature as high as +50 degrees C and its tolerance to damages due to acidic pH. The proposed method offers a rapid simple, and inexpensive means for mass screening of AchE inhibitor residues such as pesticide s and drugs in water, vegetables, and human blood serum. (C) 1997 by E lsevier Science Inc.