Ts. Lawruk et al., DEVELOPMENT OF A MAGNETIC PARTICLE-BASED ENZYME-IMMUNOASSAY FOR THE QUANTITATION OF CHLORPYRIFOS IN WATER, Journal of agricultural and food chemistry, 44(9), 1996, pp. 2913-2918
A competitive enzyme immunoassay for the quantitation of chlorpyrifos
in water that utilizes amine-terminated superparamagnetic particles as
the solid phase to covalently attach mouse monoclonal anti-chlorpyrif
os antibodies was developed. Specificity studies indicate that the mon
oclonal antibody can distinguish chlorpyrifos from its major degradati
on products, including 3,5,6-trichloro-2-pyridinol, and structurally s
imilar pesticides. The immunoassay has an estimated limit of detection
of 0.10 ng/mL in water and compares favorably with traditional chroma
tographic methods when water samples are analyzed by both methods (r =
0.985). Within- and between-assay percent coefficient of variation va
lues are <13%, and recovery of chlorpyrifos from water averaged 97% ac
ross the range of the immunoassay method.