HUMAN HPK1, A NOVEL HUMAN HEMATOPOIETIC PROGENITOR KINASE THAT ACTIVATES THE JNK SAPK KINASE CASCADE/

The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinas es (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed h ematopoietic progenitor kinase 1 (HPK1), that is expressed predominant ly in hematopoietic cells, including early progenitor cells. HPK1 is r elated distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yea st STE20 implicated in the mitogen-activated protein kinase (MAPK) cas cade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds an d phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is in hibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly , unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-bind ing domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1 activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathwa y.