CHARACTERIZATION OF A FLOW-SORTED HUMAN-CHROMOSOME-10 COSMID LIBRARY BY FISH

Fluoresence in situ hybridization (FISH) was used to localize cosmids to regions of human chromosome 10. A total of 301 cosmids were selecte d randomly from a flow-sorted human chromosome 10 cosmid library const ructed from human x hamster cell line 762-8A and arrayed in microtiter storage dishes. Over 70% (211/301) of the cosmids mapped to unique re gions of chromosome 10. About 7% (22/301) produced multiple hybridizat ion signals indicative of chimeric clones or sequences repeated at low copy number. Three cosmids (3/301, or 1%) hybridized to the centromer ic regions of chromosome 10 and one or more other human chromosomes. A bout 19% (59/301) consisted mostly or- entirely of hamster DNA inserts , and about 2% (6/301) appeared to be nonrecombinants.