EFFECT OF INTERLEUKIN-2 AND GAMMA-INTERFE RON ON RESPIRATORY BURST INMACROPHAGES - CHANGES IN THE INTRACELLULAR RADICAL PRODUCTION AND CARBOHYDRATE-SPECIFIC INTERACTIONS

In vitro generation of reactive oxygen species (ROS) by rat peritoneal macrophages stimulated with interleukin-2 (IL-2) or gamma-interferon (gamma INF) was investigated. Luminol-dependent chemiluminescence was used to measure the total (extra- and intracellular) ROS production; f luorescent due hydroethidin was used to estimate the total and intrace llular ROS generation. It was shown that pre-incubation of the cells w ith cytokines increased the contribution of intracellular ROS generati on to the total one, At the same rime, total ROS production exhibited higher sensitivity to the inhibitory effect on neoglycoconjugates (pol yacrylamide-mannose and polyacrylamide- fucose). Pre-incubation of mac rophages with gamma INF or IL-2 produced no effect on the neoglycoconj ugate binding to the cell membrane. Combination of cytokines with neog lycoconjugates for macrophages pre-stimulation produced pronounced inh ibitory effect on the generation activated either by phorbolmyristatac etate or opsonised zymosan.