Na. Kalabina et al., MIXED LIPID-PROTEIN FILMS OF BACTERIAL PH OTOSYNTHETIC REACTION CENTERS .1. INVESTIGATION OF MIXED MONOLAYERS AT THE AIR WATER INTERFACES/, Biologiceskie membrany, 13(4), 1996, pp. 410-422
Mixed lipid-protein monolayers composed of reaction center (RC) protei
ns and lipids were investigated. RCs were isolated from Chloroflexus a
urantiacus and Rhodobacter sphaeroides (wild type) photosynthetic bact
eria and mixed with natural and synthetic lipids. The optimal subphase
conditions for forming monolayers composed of phosphatidylcholine - R
C (1:26. M/M) and phosphatidylethanolamine - RC (1:26, MFI) were deter
mined. The optimal parameters of the water subphase were found to be 1
0 mM phosphate buffer (pH 7,0) and 20 degrees C. The monolayers were t
ested for their stability under constant surface pressure.