CLONING AND CHROMOSOMAL LOCALIZATION OF THE RAT STAF5 AND E-YY1 GENES

Rat beta-casein gene expression is controlled in part by the positivel y acting factor Stat5 and the negatively acting factor Yy1. Partial cD NAs encoding the SH2 domain of rat Stat5a and Stat5b and the zinc fing er domain of rat Yy1 have been cloned by an RT-PCR based approach. The nucleotide sequences encoding the SH2 domain of rat Stat5a and Stat5b 75 % homologous. The rat Yy1 cDNA is 95 % homologous to its human and mouse counterparts in the zinc finger domain-encoding region. Using f luorescence in situ hybridization, we have localized Stat5a to rat chr omosome band 10q32.1 and Yy1 to rat chromosome band 6q32.