H. Hayashida et al., MOLECULAR ANALYSIS OF A NEW INSERTION-SEQUENCE FROM ACTINOBACILLUS (HAEMOPHILUS) ACTINOMYCETEMCOMITANS FDC Y4, Microbiology, 142, 1996, pp. 2449-2452
We have found a new insertion sequence (IS), designated ISAa1, downstr
eam of the S10 operon in Actinobacillus (Haemophilus) actinomycetemcom
itans FDC Y4. ISAa1, the first IS element characterized in this organi
sm, is 705 bp long and lacks terminal inverted repeats. This element d
isplayed significant homology with IS200. Hybridization patterns of ge
nomic DNA of seven A. actinomycetemcomitans strains with an internal I
SAa1 probe varied depending on the serotypes, suggesting that ISAa1 mi
ght be a useful tool for epidemiological studies.