T. Yokota et al., HYALURONIC-ACID SYNTHESIS BY THE MALIGNANT FIBROUS HISTIOCYTOMA CELL-LINE NMSG-10 IN-VITRO AND ITS LOCALIZATION, Pathobiology, 64(2), 1996, pp. 67-72
Viscous material secreted from NMSG 10 cells cultured from malignant f
ibrous histiocytoma of human bone was demonstrated using two-dimension
al electrophoresis to be glycosaminoglycans (GAGs) consisting of mainl
y hyaluronic acid (HA), along with small amounts of heparan sulfate (H
S) and chondroitin sulfate (ChS). The biosynthesis of HA in a cell-fre
e system was located at the plasma membrane fraction by colloidal-iron
-reactive ultrastructural staining. The molecular weight of the HA syn
thesized by the plasma membrane fraction was estimated to be about 90,
000 D by gel filtration. The material secreted by the original tumor t
issue of NMSG 10 cells was demonstrated to be GAGs consisting of HA, H
S, ChS and dermatan sulfate using two-dimensional electrophoresis. The
se findings suggest that transformed tumor cells could synthesize HA a
t the plasma membrane and release it into the stroma of tumor tissue.