PEPTIDE CONTENT-DETERMINATION OF CRUDE SYNTHETIC PEPTIDES BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY AND NITROGEN-SPECIFIC DETECTION WITH A CHEMILUMINESCENT NITROGEN DETECTOR

The need for a rapid and accurate method to assess peptide content is driven by the recent surge in demand for pharmaceutical-grade syntheti c peptides manufactured by solid-phase chemistry. Routinely, the purit y of synthetic peptides is analyzed by reversed-phase HPLC with UV det ection, and peptide content is typically determined from amino acid an alysis of synthetic peptides. Recently, we developed a new method to a ssess the peptide content of synthetic peptides in one simple techniqu e: simultaneous reversed-phase HPLC-chemiluminescent nitrogen detectio n and UV detection. In this paper we present the simplicity of this me thod and its universal application to analyzing crude synthetic peptid es produced by solid-phase synthesis, an important first step in manuf acturing bioactive peptides.