This paper describes the execution of enzyme-linked immunosorbent assa
ys (ELISA), (i) in immunosorbent columns with antibodies immobilized o
n porous particles, (ii) where samples and reagents are metered by val
ves and syringe pumps, (iii) samples, reagents, substrate, and wash bu
ffers are transported into or through the system by high pressure liqu
id chromatography pumps, and (iv) enzyme reaction product is detected
by absorbance. The normal protocol used for ELISA was modified in that
antigen was complexed with enzyme conjugated antibody in the autosamp
ler and an aliquot introduced into the system where it was transported
to the immunosorbent and captured. Substrate was subsequently pumped
into the immunosorbent bed and the product swept to an absorbance dete
ctor for quantitation.