DETERMINATION OF FOSFOMYCIN IN HUMAN URINE BY CAPILLARY GAS-CHROMATOGRAPHY - APPLICATION TO CLINICAL PHARMACOKINETIC STUDIES

Authors
DIOSVIEITEZ MC GONI MM RENEDO MJ FOS D
Citation
Mc. Diosvieitez et al., DETERMINATION OF FOSFOMYCIN IN HUMAN URINE BY CAPILLARY GAS-CHROMATOGRAPHY - APPLICATION TO CLINICAL PHARMACOKINETIC STUDIES, Chromatographia, 43(5-6), 1996, pp. 293-295
Citations number
7
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
43
Issue
5-6
Year of publication
1996
Pages
293 - 295
Database
ISI
SICI code
0009-5893(1996)43:5-6<293:DOFIHU>2.0.ZU;2-L
Abstract
A capillary gas chromatographic method for the determination of fosfom ycin in human urine is described. After dilution of the sample and der ivatization, analysis was on a HP-1 capillary column and a flame ioniz ation detector was used to determine the bistrimethylsilyl derivative of fosfomycin. Response was linear in the range 50-5000 mu g mL(-1). T he detection limit was about 10 mu g mL(-1). The within and between da y coefficients of variation did not exceed 6 %. The method was applied to the determination of fosfomycin in urine samples collected during clinical pharmacokinetic studies.