COMPARISON OF SUPERCRITICAL CHF3 AND CO2 AND METHANOL-MODIFIED CHF3 AND CO2 FOR EXTRACTION OF SULFONAMIDES FROM CHICKEN LIVER

Supercritical CHF3 and methanol-modified CHF3 were compared with super critical CO2 and methanol-modified CO2 for extraction of sulfonamides from fortified chicken liver admired with Hydromatrix. Results showed that solvating power and selectivity were higher for supercritical and methanol-modified CHF3 than for supercritical and methanol-modified C O2. Visual observation showed that chicken liver extract obtained with methanol-modified CHF3 was cleaner than that obtained with methanol-m odified CO2. Fat precipitated in the solvent trap when CO2 was used as the extraction medium. Also, simple off-line collection of fortified chicken liver extract obtained with CO2 in a solid-phase extraction ca rtridge (packed with either C-18 or alumina) followed by phosphate buf fer-methanol (50 + 50) rinse yielded an extract that required no furth er cleanup for analysis.