VERO CELL-LINE (B) FOR BIOMEDICAL PREPARA TIONS

Citation
Ry. Podchernyayeva et al., VERO CELL-LINE (B) FOR BIOMEDICAL PREPARA TIONS, Voprosy virusologii, 41(4), 1996, pp. 183-185
Citations number
7
Categorie Soggetti
Virology
Journal title
ISSN journal
05074088
Volume
41
Issue
4
Year of publication
1996
Pages
183 - 185
Database
ISI
SICI code
0507-4088(1996)41:4<183:VC(FBP>2.0.ZU;2-L
Abstract
A variant of Vero cell line has been obtained, adapted to the Russian Eagle's medium with 8% fetal calf serum. The variant is characterized by intensive cell proliferation, possesses a different content of chro mosomes, contains no oncogens and contaminants, specifically, Mycoplas ma. The strain was characterized and certified in accordance with the WHO requirements. Inoculation (110 ampules) and working (100 ampules) stocks at the levels of the 170th and 178th passages were placed for s torage in liquid nitrogen. Vero (B) cell line is sensitive to herpes s implex virus types 1 and 2, CMV, hepatitis A virus, recombinant variol ovaccinia strain expressing HbS antigen, etc. The line retains its bio logical properties from passage 178 to passage 200 and is recommended for control and preparation of various biomedical agents, including an tiviral vaccines.