ILLEXIN-A, ILLEXIN-B, AND ILLEXIN-C FROM SQUID INK SHOULD HAVE A BRANCHED STRUCTURE

Citation
Y. Takaya et al., ILLEXIN-A, ILLEXIN-B, AND ILLEXIN-C FROM SQUID INK SHOULD HAVE A BRANCHED STRUCTURE, Biochemical and biophysical research communications, 226(2), 1996, pp. 335-338
Citations number
4
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
226
Issue
2
Year of publication
1996
Pages
335 - 338
Database
ISI
SICI code
0006-291X(1996)226:2<335:IIAIFS>2.0.ZU;2-Q
Abstract
Ilexin A, illexin B, and illexin C, isolated from ink of the squid Ill ex argentinus, have been reported to have a structure consisting of li near repeating units of (-6GalNAc alpha 1-3GlcA beta 1-3Fuc alpha 1-)( n) [Biochem. Biophys. Res. Commun. 198, 560 (1994)]. This structure wa s further investigated using NOESY and atmospheric pressure MS/MS meas urement of PA-trisaccharide obtained by acid hydrolysis of illexin C. The results indicated that the structure is, in fact, branched. Observ ation of both [GalNAc-Fuc-PA](+) and [GlcA-Fuc-PA](-) obtained as prod uct ions from the molecular ion of PA-trisaccharide on the MS/MS spect ra suggested a branched repeating unit. NOEs between H-1 of GalNAc and H-3 of Fuc, and between H-1 of GlcA and H-4 of Fuc, revealed the posi tions of glycosidic linkages, and from the results of sodium periodate oxidation of illexin C and PA-trisaccharide, the structure was determ ined to be (-3GlcA beta 1-4(GalNAc alpha 1-3)Fuc alpha 1-}(n). (C) 199 6 Academic Press, Inc.