AGE-RELATED-CHANGES IN THE OVALBUMIN GENE OF THE JAPANESE-QUAIL

Northern hybridization studies showed that the level of ovalbumin mRNA decreases in the oviduct of the Japanese quail after adulthood. In or der to find out if this is due to changes in the conformation of the c hromatin containing the promoter region of the gene, nuclei of the ovi duct of young, adult and old birds were digested by DNaseI and microco ccal nuclease (MNase). Southern hybridization with the labelled promot er showed that this region is less sensitive to the two enzymes in the old birds. Both the endonucleases recognized the same hypersensitive sites. The results indicate that the chromatin containing the promoter is present in an open conformation in adult birds. Gel mobility shift assay using a 20-mer dsDNA containing the CAAT-box and nuclear extrac t of oviduct shows the presence of trans-acting factors that bind to t his region. The levels of these factors are lower in old birds. This m ay be the reason for the lower expression of the gene in old birds. (C ) 1996 Academic Press, Inc.