Jt. Mao et al., COCAINE DOWN-REGULATES IL-2-INDUCED PERIPHERAL-BLOOD LYMPHOCYTE IL-8 AND IFN-GAMMA PRODUCTION, Cellular immunology, 172(2), 1996, pp. 217-223
Cocaine has multiple immunomodulatory effects, including the ability t
o influence cytokine release in immunoeffector cells. Little is known,
however, regarding the effects of cocaine on cytokine production by h
uman peripheral blood lymphocytes (PBL), The effect of cocaine on PBL
cytokine profiles and the molecular mechanisms responsible for the mod
ulation of cytokine mRNA expression were investigated. To evaluate the
effects of cocaine on cytokine production, conditioned supernatant fr
om IL-2-stimulated PBL was evaluated by cytokine-specific ELISA (IL-4,
IL-5, IL-8, IL-10, IFN-gamma, and TGF-beta) following in vitro cocain
e exposure. Cocaine abrogated the IL-a-induced production of IFN-gamma
and IL-8 in a dose-responsive manner. Cocaine also decreased PBL IFN-
gamma and IL-8 mRNA expression as determined by Northern blot and slot
blot analysis. Cocaine did not affect the stability of the IFN-gamma
and IL-8 mRNA. Nuclear run-on assays revealed that cocaine down-regula
ted the rate of IFN-gamma and IL-8 transcription. These findings sugge
st that the immunomodulatory effects of cocaine may be mediated, in pa
rt, by modification of lymphocyte cytokine production. (C) 1996 Academ
ic Press, Inc.