COCAINE DOWN-REGULATES IL-2-INDUCED PERIPHERAL-BLOOD LYMPHOCYTE IL-8 AND IFN-GAMMA PRODUCTION

Citation
Jt. Mao et al., COCAINE DOWN-REGULATES IL-2-INDUCED PERIPHERAL-BLOOD LYMPHOCYTE IL-8 AND IFN-GAMMA PRODUCTION, Cellular immunology, 172(2), 1996, pp. 217-223
Citations number
47
Categorie Soggetti
Cell Biology",Immunology
Journal title
ISSN journal
00088749
Volume
172
Issue
2
Year of publication
1996
Pages
217 - 223
Database
ISI
SICI code
0008-8749(1996)172:2<217:CDIPLI>2.0.ZU;2-U
Abstract
Cocaine has multiple immunomodulatory effects, including the ability t o influence cytokine release in immunoeffector cells. Little is known, however, regarding the effects of cocaine on cytokine production by h uman peripheral blood lymphocytes (PBL), The effect of cocaine on PBL cytokine profiles and the molecular mechanisms responsible for the mod ulation of cytokine mRNA expression were investigated. To evaluate the effects of cocaine on cytokine production, conditioned supernatant fr om IL-2-stimulated PBL was evaluated by cytokine-specific ELISA (IL-4, IL-5, IL-8, IL-10, IFN-gamma, and TGF-beta) following in vitro cocain e exposure. Cocaine abrogated the IL-a-induced production of IFN-gamma and IL-8 in a dose-responsive manner. Cocaine also decreased PBL IFN- gamma and IL-8 mRNA expression as determined by Northern blot and slot blot analysis. Cocaine did not affect the stability of the IFN-gamma and IL-8 mRNA. Nuclear run-on assays revealed that cocaine down-regula ted the rate of IFN-gamma and IL-8 transcription. These findings sugge st that the immunomodulatory effects of cocaine may be mediated, in pa rt, by modification of lymphocyte cytokine production. (C) 1996 Academ ic Press, Inc.