HUMAN INTERFERON REDUCES SURFACE EXPRESSION BUT NOT TOTAL PRODUCTION OF HERPES-SIMPLEX VIRUS TYPE-1 GLYCOPROTEINS GC AND GE IN HETEROLOGOUSHAMSTER-CELLS

The effect of interferon (IFN) on herpes simplex virus type 1 (HSV-1)- induced glycoproteins gC and gE was investigated in a heterologous IFN /cell model, In this model, the effect on surface expression of the gl ycoproteins could be studied separately from the effect on virus multi plication, Pretreatment of baby hamster kidney cells (BHK) with hetero logous human leukocyte IFN suppressed surface expression of HSV-l-enco ded gC and gE but had no influence on total production of the glycopro teins, This was in contrast to the effect on human embryonic fibroblas t cells (HE) (homologous IFN and cells), where surface expression as w ell as total production of glycoproteins were reduced, The surface exp ression was demonstrated by antibody-sensitized monodisperse polystyre ne beads, and immunoblotting and two-dimensional electrophoretic analy sis of radioisotope-labeled proteins were used to study the total prod uction.