METABOLISM OF META-CHLORINATED OR AND PARA-CHLORINATED BENZOIC-ACIDS BY THE CRUDE EXTRACT OF ALCALIGENES SP STRAIN CPE3 GROWN ON 3,4-DICHLOROBENZOIC ACID/

A crude extract of Alcaligenes sp. CPE3 strain grown on 3,4-dichlorobe nzoic acid metabolised 3- and 4-chlorobenzoic acid by reactions requir ing O-2 and NADH, and 3,4-dichlorobenzoic acid by a reaction requiring O-2, NADH, FAD and FMN. The specific activity of the extract vs. 3-ch lorobenzoic acid was described by the Michaelis-Menten kinetics, that vs. 4-chlorobenzoic acid was described by a substrate inhibitory kinet ics and that vs. 3,4-dichlorobenzoic acid exhibited a two-peaked profi le.