FEEDING OF NUTRIENTS DELAYS APOPTOTIC DEATH IN FED-BATCH CULTURES OF RECOMBINANT NS0 MYELOMA CELLS

The addition of concentrated nutrient solutions in fed-batch cultures of recombinant GS-NSO cells delays the onset of apoptosis by 24 to 48 hours and decreases the specific rate of apoptotic death approximately two-fold. This decrease in apoptosis appears primarily responsible fo r the increase in culture longevity that results in five- to ten-fold increases in final product concentrations in fed-batch cultures.