Lr. Falk et Pm. Doran, INFLUENCE OF INOCULUM MORPHOLOGY ON GROWTH OF ATROPA-BELLADONNA HAIRYROOTS AND PRODUCTION OF TROPANE ALKALOIDS, Biotechnology letters, 18(9), 1996, pp. 1099-1104
The specific growth rate of Atropa belladonna hairy roots measured in
terms of root length increased by about 25% to 0.49 d(-1) when the ino
culum consisted of a root with the primary root tip excised. The bioma
ss dry weight produced after 14d increased by 28%. Tn contrast, presen
ce of laterals in the inoculum with or without lateral tips excised di
d not influence the growth rate. Although hyoscyamine was found to acc
umulate at higher concentrations in the more mature root tissues (2.1
+/- 0.2 mg g(-1)) than in the tips (1.1 +/- 0.3 mg g(-1)), hyoscyamine
content in the harvested roots was independent of inoculum morphology
.