COUPLING OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH RAMAN-SPECTROMETRY

Raman spectrometry has been tested as a method for detection in HPLC. Using a laboratory-made cell similar to classical UV cells, toluene an d naphthalene have been analyzed as solutions in acetonitrile-water (7 5:25 or 95:5 v/v) mixtures. Using a monochannel detection, a detection limit of about 480 mg/l (9.6 mu g for the 20-mu l injection loop) for toluene has been found and the possibility to distinguish between co- eluted toluene and naphthalene and to make a quantitative analysis has been proved.