MOLECULAR ANALYSIS AND POLYMORPHISM OF THE DLA-DQA GENE

A full-length cDNA clone and two overlapping genomic clones correspond ing to the canine DQA class II gene were isolated and sequenced. Restr iction mapping and sequence data allow identification and orientation of the five exons corresponding to the alpha (alpha) chain. Sequence a nalysis of exon 2 amplified from 17 unrelated dogs of various breeds i dentified seven alleles. The structure of the canine DQA gene is simil ar to HLA-DQA1 and other mammalian DQA genes. This study will serve as a reference for developing a typing system for the DLA-DQA gene for d onor and recipient matching in the canine model for organ and bone mar row transplantation.