STRATEGY AND DESIGN OF NOVEL REAGENTS FOR THE FLUOROMETRIC ANALYSIS OF BIOMOLECULES

This article covers molecular designs to develop several new fluoromet ric reagents and their applications to increase the sensitivities up t o the picomole level using HPLC for the measurement of biomolecules. T he methods were designed to demonstrate the physiological activities, for example (1) N-(9-acridinyl)maleimide (NAM) for the measurement of SN, -S-S-, and sulfite such as cysteine, (2) diphenyl-1-pyrenylphosphi ne (DPPP) for the hydroperoxides in lipids, serum, tissues, and foodst uffs, (3) 9-bromomethylacridine (9-BrMA), (4) thracene-2,3-dicarboxyli mide)ethyltrifluoromethane sulfonate (AE-OTf) for carboxylic acids, an d (5) The chiral fluorometric labelling reagent -tert-butyl-2-methyl-1 ,3-benzodioxole-4-carboxylic acid (TBMB) to identify the chiralities o f amino acids, sugars, and mono- and diacylglycerols.