PURIFICATION AND CHARACTERIZATION OF A LECTIN FROM AMARANTHUS-HYPOCHONDRIACUS VAR MEXICO SEEDS

Authors
OZEKI M KAMEMURA K MORIYAMA K ITOH Y FURUICHI Y UMEKAWA H TAKAHASHI T
Citation
M. Ozeki et al., PURIFICATION AND CHARACTERIZATION OF A LECTIN FROM AMARANTHUS-HYPOCHONDRIACUS VAR MEXICO SEEDS, Bioscience, biotechnology, and biochemistry, 60(12), 1996, pp. 2048-2051
Citations number
18
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
12
Year of publication
1996
Pages
2048 - 2051
Database
ISI
SICI code
0916-8451(1996)60:12<2048:PACOAL>2.0.ZU;2-P
Abstract
A lectin from Amaranthus hypochondriacus var, Mexico (AHML) was purifi ed by affinity chromatography using asialofetuin-Sepharose 4B, AHML is specific for N-acetyl-D-galactosamine as are the other Amaranthus lec tins, AHML has no carbohydrate moiety and requires no metal ion for th e hemagglutination activity. The pI of AHML, is 6.8, AHML has a native molecular mass of 45.0 kDa and is composed of homo-subunits having mo lecular masses of 36.8 kDa.