INTERFERENCE OF HEPARIN IN PLASMA LEVOCARNITINE DETERMINATION WITH RADIOENZYME ASSAY

Authors
MARZO A CARDACE G CORBELLETTA C VERGA P VINCIGUERRA C
Citation
A. Marzo et al., INTERFERENCE OF HEPARIN IN PLASMA LEVOCARNITINE DETERMINATION WITH RADIOENZYME ASSAY, Arzneimittel-Forschung, 45-2(12), 1995, pp. 1303-1305
Citations number
12
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
Arzneimittel-Forschung → ACNP
ISSN journal
00044172
Volume
45-2
Issue
12
Year of publication
1995
Pages
1303 - 1305
Database
ISI
SICI code
0004-4172(1995)45-2:12<1303:IOHIPL>2.0.ZU;2-F
Abstract
This paper describes the interference of heparin (CAS 9005-49-6) in wh ole plasma used for free and total levo-carnitine (L-carnitine, CAS 54 1-15-1) analysis Alkaline hydrolysis required for total L-carnitine me asurement does not overcome the problem. The same interference is also present in long-chain acyl-L-carnitine assay, because heparin precipi tates together with proteins and long-chain esters of L-carnitine in t he acid insoluble fraction of perchloric acid treated plasma sample In this case an appropriate cleaning of the sample is recommended before the assay.