MONOCLONAL-ANTIBODIES TO CHICKEN OVOTRANSFERRIN - EPITOPIC AND PHYLOGENETIC ANALYSIS

1. The ability of four domain-specific anti-chicken ovotransferrin ant ibodies to bind to turkey, quail, duck, pheasant and goose ovotransfer rins was examined directly by an enzyme-linked immunoassay and indirec tly in a competitive radioimmunoassay. 2. The ability of these same ov otransferrins to compete with radioiodinated chicken ovotransferrin fo r binding to transferrin receptors on chick embryo red blood cells was also tested. 3. The hypothesis that evolutionarily conserved determin ants involved in receptor recognition can be predicted by monoclonal a ntibodies that block binding of ovotransferrin to receptor was found t o be incorrect.