ENZYME-REACTIONS IN THE PRESENCE OF CYCLODEXTRINS - BIOSENSORS AND ENZYME ASSAYS

Citation
Jht. Luong et al., ENZYME-REACTIONS IN THE PRESENCE OF CYCLODEXTRINS - BIOSENSORS AND ENZYME ASSAYS, Trends in biotechnology, 13(11), 1995, pp. 457-463
Citations number
26
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
ISSN journal
01677799
Volume
13
Issue
11
Year of publication
1995
Pages
457 - 463
Database
ISI
SICI code
0167-7799(1995)13:11<457:EITPOC>2.0.ZU;2-T
Abstract
Cyclodextrins, macrocyclic carbohydrates with apolar internal cavities , can form complexes with, and solubilize many normally water-insolubl e compounds. Ferrocene and its derivatives, tetrathiafulvalene and tet ramethylbenzidine, can function as redox mediators, but are insoluble in water; when they are complexed with cyclodextrins, they can be used in enzymatic assays and in the construction of mediated biosensors. I n addition, the solubilization of polynuclear aromatic hydrocarbons (P AHs), including the potent carcinogen benzo[a]pyrene, by cyclodextrins has enabled the detection of these important environmental contaminan ts.